Knockout! A CRISPR/Cas Gene Targeting Lab – miniPCR bio

This lab is easy for teachers to prepare, makes gene targeting accessible to students, and packs a powerful punch! Become a genetic engineer and use CRISPR/Cas to disable a gene in bacteria. Scientists use the CRISPR/Cas system to alter the DNA in living cells. Now…
Perform cutting-edge genome editing! This lab allows advanced students to use the CRISPR/Cas system to disrupt a gene in bacteria and observe the resulting phenotypic change. 

The ABCs of CRISPR in Tephritidae: developing methods for inducing heritable mutations in the genera Anastrepha, Bactrocera and Ceratitis - Sim - 2019 - Insect Molecular Biology - Wiley Online Library

Perform cutting-edge genome editing! This lab allows advanced students to use the CRISPR/Cas system to disrupt a gene in bacteria and observe the

Knockout! A CRISPR/Cas Gene Targeting Lab

miniPCR

Gene Knockout

Resources: Workshop Report: Summer 2020 Virtual CRISPR in the Classroom

CRISPR/Cas – miniPCR bio

MLH1 CRISPR Knockout and Activation Products (m)

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Introducing Chopped! A CRISPR/Cas Lab from miniPCR bio™

Lab 8 CRISPR.docx - Lab 7: Transformation with CRISPR Knockout Objectives 1 Create a knockdown mutant of E. coli using the CRISPR/Cas9 gene editing