Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

Cells, Free Full-Text

Label-free, full-field visualization of red blood cell (RBC)

Serial Number Alcohol 120 1.9 6 - Colaboratory

Remote immune processes revealed by immune-derived circulating cell-free DNA

Cell-free expression and synthesis of viruses and bacteriophages: applications to medicine and nanotechnology - ScienceDirect

Cell-free mutant analysis combined with structure prediction of a lasso peptide biosynthetic protein B2

THE CELL : PAUL REVERE11 : Free Download, Borrow, and Streaming : Internet Archive

Sequencing of Circulating Cell-free DNA during Pregnancy

Cell-Free Gene Expression: Methods and Protocols