Cells, Free Full-Text

Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.

Cells, Free Full-Text

Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C - ScienceDirect

Cells, Free Full-Text

Textile-embedded cell-free biosensors

Cells, Free Full-Text

Nt Novo Cella Get File - Colaboratory

Remote immune processes revealed by immune-derived circulating cell-free DNA

Nature Reviews Molecular Cell Biology on X: From May cover Challenges and directions in studying cell–cell communication by #ExtracellularVesicles @DaveCarter1234 @guillaume_niel @VaderPieter Clayton Lambert & Raposo #EVsAreCool FREE pdf: https

Cancers, Free Full-Text

Cell-Free Protein Synthesis: Methods and Protocols

Cell-free mutant analysis combined with structure prediction of a lasso peptide biosynthetic protein B2