JoF, Free Full-Text

Paracoccidioidomycosis (PCM) is a systemic mycosis caused by Paracoccidioides brasiliensis, a thermally dimorphic fungus, which is the most frequent endemic systemic mycosis in many Latin American countries, where ~10 million people are believed to be infected. In Brazil, it is ranked as the tenth most common cause of death among chronic infectious diseases. Hence, vaccines are in development to combat this insidious pathogen. It is likely that effective vaccines will need to elicit strong T cell-mediated immune responses composed of IFNγ secreting CD4+ helper and CD8+ cytolytic T lymphocytes. To induce such responses, it would be valuable to harness the dendritic cell (DC) system of antigen-presenting cells. To assess the potential of targeting P10, which is a peptide derived from gp43 secreted by the fungus, directly to DCs, we cloned the P10 sequence in fusion with a monoclonal antibody to the DEC205 receptor, an endocytic receptor that is abundant on DCs in lymphoid tissues. We verified that a single injection of the αDEC/P10 antibody caused DCs to produce a large amount of IFNγ. Administration of the chimeric antibody to mice resulted in a significant increase in the levels of IFN-γ and IL-4 in lung tissue relative to control animals. In therapeutic assays, mice pretreated with αDEC/P10 had significantly lower fungal burdens compared to control infected mice, and the architecture of the pulmonary tissues of αDEC/P10 chimera-treated mice was largely normal. Altogether, the results obtained so far indicate that targeting P10 through a αDEC/P10 chimeric antibody in the presence of polyriboinosinic: polyribocytidylic acid is a promising strategy in vaccination and therapeutic protocols to combat PCM.

Flow chart for selection of research articles.

Letterhead Fonts / LHF Jalopy Joe / Period Fonts

IJMS, Free Full-Text

The Imaginary Journal of Poetic Economics: The impact of funding agency open access policies (March 31, 2012 Dramatic Growth of Open Access)

Nutrients, Free Full-Text

Healthcare, Free Full-Text

Term Papers Online: 90+ FREE Websites for Your Paper

What Is Indexed Universal Life Insurance (IUL)?

Viruses, Free Full-Text

Jofi Font

From the Free Collections drop down menu, you can access other free e-journal gateways and be able to obtain full-text articles. We will examine one of. - ppt download

Nathan Fernance - Sports Physiotherapist - ATUNE Health Centres

JoVE Peer Reviewed Scientific Video Journal - Methods and Protocols

IJMS, Free Full-Text