Pathogens, Free Full-Text
Por um escritor misterioso
Last updated 19 março 2025

In Brazil, blood donation is regulated by the Brazilian Ministry of Health, and all States follow the same protocol for clinical and laboratory screening. Brazil is an endemic country for Chagas disease (CD), caused by Trypanosoma cruzi, and for leishmaniasis, caused by a species of Leishmania spp. Screening for leishmaniosis is not routinely performed by blood banks. Given the antigenic similarity between T. cruzi and Leishmania spp., cross-reactions in serological tests can occur, and inconclusive results for CD have been found. The objective of this study was to apply molecular techniques, e.g., nPCR, PCR, and qPCR, to clarify cases of blood donation candidates with non-negative serology for CD and to analyze the difference between the melting temperature during real-time PCR using SYBR Green. Thirty-seven cases that showed non-negative results for CD using chemiluminescent microparticle immunoassay (CMIA) tests from blood banks in Campo Grande, MS, and Campinas, SP, were analyzed. In the serum samples, 35 samples were evaluated by ELISA, and 24.3% (9/35) showed positive results for CD. nPCR was able to detect 12 positive results in 35 samples (34.28%). qPCR for T. cruzi was quantifiable in the samples that showed a value ≥0.002 par eq/mL (parasite equivalents per milliliter), and in 35 samples, 11 (31.42%) were positive. Of all evaluated samples using the described tests (CMIA, ELISA, nPCR, and qPCR), 18 (48.6%) were positive for CD. For MCA by qPCR, the melting temperature was 82.06 °C ± 0.46 for T. cruzi and 81.9 °C ± 0.24 for Leishmania infantum. The Mann–Whitney test showed a significant value of p < 0.0001. However, the differentiation between T. cruzi and L. infantum could not be considered due to temperature overlap. For leishmaniasis, of the 35 samples with non-negative serology for CD tested by the indirect fluorescent antibody test (IFAT), only one sample (2.85%) was positive (1:80). The PCR for Leishmania spp. was performed on 36 blood samples from donation candidates, and all were negative. qPCR for L. infantum showed 37 negative results for the 37 analyzed samples. The data presented here show the importance of performing two different tests in CD screening at blood banks. Molecular tests should be used for confirmation, thereby improving the blood donation system.

Sensitive detection of a bacterial pathogen using allosteric probe-initiated catalysis and CRISPR-Cas13a amplification reaction

Features of pathogens. There are various types of pathogens including

Parasitologia E Micologia Humana Download - Colaboratory

Pathogens, Free Full-Text

Bloodborne Pathogens Handouts, First Aid

Bacterial Invasion: The Paradigms of Enteroinvasive Pathogens

Detection of Viable Pathogens Using Label-Free Electrical Detection of Nucleic Acid Amplification : LIBNA

Pathogens, Free Full-Text

Pathogens Packet - Bacteria Viruses Protozoa Fungi Parasites and Prions - Homeschool Den

Evolution of pathogen tolerance and emerging infections: A missing experimental paradigm

Lg Download Software V1.3.6.3 - Colaboratory
Bacterial Fish Pathogens: Disease of Farmed and Wild Fish

Cell Host & Microbe on X: Microbiota-Driven Firewall Stops Pathogens: @BraedonMcdonald & Co show that pathogen clearance by Kupffer cells is governed by #gut #microbiota & D-lactate, which reaches liver via portal

Cellular Microbiology - Wiley Online Library

The influence of probiotic bacteria and human gut microorganisms causing opportunistic infections on Blastocystis ST3, Gut Pathogens
Recomendado para você
-
Boletim Mercado Financeiro – DGBB Comunicação e Estratégia19 março 2025
-
Boletim Focus: projeção de inflação e dólar para 2023 recua; estimativas de IPCA para 2024 e PIB sobem19 março 2025
-
Ler ou não ler o boletim Focus? Eis a questão - Inteligência Financeira19 março 2025
-
T8986 - Boletim Informativo FENASEG - 238/260_1974 by CNseg - Issuu19 março 2025
-
Frontiers Cover Crops and Mechanical Scarification in the Yield and Industrial Quality of Upland Rice19 março 2025
-
O que é o boletim Focus? - Glossário - Inteligência Financeira19 março 2025
-
tecbond MF quartzolit19 março 2025
-
Mercado estima Selic em 6,25% e inflação de 5,82% em 202119 março 2025
-
Engates com Rosca - Stucchi19 março 2025
-
Arrecadação extra com voto de qualidade do Carf pode ser menor que esperada pelo governo19 março 2025
você pode gostar
-
E-Ticket: By Hook Or By Rook19 março 2025
-
17 Best Michaels Coupon ideas michaels coupon, printable coupons, coupons19 março 2025
-
CHEGOU NOVO CODIGUIN FFWS! VEJA COMO RESGATAR E O QUE VEM NO FREE FIRE WORLD SERIES codigo infinito19 março 2025
-
PSA: King of Fighters '97 sneaks onto iOS, Android devices19 março 2025
-
trdwll — Days Gone19 março 2025
-
Jacob Zuma (President of South Africa) - On This Day19 março 2025
-
Anderlecht vs West Ham Prediction, Betting Tips & Odds │6 OCTOBER19 março 2025
-
2023-2024 Fk Crvena zvezda Home Concept Football Shirt19 março 2025
-
BINGRAN OnePlus 9 Pro Case,Shockproof Slim TPU 36019 março 2025
-
DAMASCO SECO FELIZ 150GR – SUPERMERCADO NICOLINI ON-LINE19 março 2025